Fig. 8
The effect of 60 % ASMDM on the ability of B. cenocepacia to invade the HLCC monolayer. Confluent HLCCs were exposed to 60 % ASMDM diluted in Ham’s F-12 complete medium, infected with B. cenocepacia at MOI 5 or mock-infected with saline, and incubated at 37 °C (7 % CO2). After 24 h, HLCCs were washed and then lysed with sterile dH2O. The bacterial density (CFU/mL) in each lysate was compared to the starting bacterial density at T0. Differences in mean ratio of CFU/mL were significantly different as indicated by oneway ANOVA (p = 0.0004). A Tukey-HSD post-hoc test indicated that the mean ratio CFU/mL in infected HLCCs + 60 % ASMDM is significantly different than in infected or mock-infected HLCCs + medium and in mock-infected HLCCs + 60 % ASMDM (p = 0.0007, p = 0.0007, and p = 0.0013, respectively). Values are averages of three independent experiments with single wells. Bars show standard deviation. Variables with different letters are statistically different (oneway ANOVA, p < 0.05)