Fig. 6

PLNC8 αβ binds to P. gingivalis in a dose-dependent manner. Binding of P. gingivalis to PLNC8 αβ and to anti- P.gingivalis antibodies was analyzed by SPR. Both P. gingivalis ATCC 33277 (a) and W50 (b) were found to bind to immobilized PLNC8 αβ (280 nM). The binding was verified by pre-incubating the bacteria with different concentrations of soluble PLNC8 αβ prior to analysis, which resulted in a significantly reduced binding to the immobilized bacteriocins. Pre-incubation of P. gingivalis ATCC 33277 (c) and W50 (d) with increasing concentrations of soluble PLNC8 αβ prior to analysis reduced the bacterial binding to anti-P. gingivalis antibodies in a dose-dependent manner. Results are presented from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001