Fig. 4

Dose- and time dependent release of CF in response to bacteriocin PLNC8 αβ. a Release of 5 (6)-carboxyfluorescein (CF) from liposomes (5:95 POPS: POPC) after 30 min with addition of PLNC8 α (red), β (blue) and αβ 1:2 (green). The interaction kinetics was recorded every minute for PLNC8 α (b) and PLNC8 β (c) with the liposomes and is displayed in increasing bacteriocin concentration (0.005–2 μM) indicated by the arrow. The total lipid concentration was kept constant at 25 μM. CF release without addition of bacteriocins were < 1 % and all data points are average of n = 3. The thick red and blue lines in B and C indicate the highest peptide concentration