Fig. 4
From: Bacillus anthracis S-layer protein BslA binds to extracellular matrix by interacting with laminin
Identification of the interaction between BslA and laminin. a Dot Blot (1, 2, 3, ECM incubated with BslA(260–652), triplicate dot; 4,5, 6, ECM incubated with sonicated E. coli BL21(DE3) triplicate dot). The result of the overlay suggested that the ligand(s) of BslA are located in the ECM component. b Far Western blot. Both non- pretreated ECM (lane 1 and lane 4) and collagenase-digested ECM (lane 2, 3, 5) were used for Far Western blot test. The proteins indicated by arrows were analyzed by MS. c Far Western blot using laminin as target protein. Lane 1, both BslA(260-652) and polyclonal BslA antibody was added regularly. Lane 2, only the polyclonal BslA serum was added, negative control. d Flow cytometry analysis of the blocking effect of anti-laminin polyclonal antibodies. Cells were preincubated with laminin-specific antibodies (1:50 dilution) or naive rabbit sera for 1 h prior to incubation with BslA(260–652)