Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates

Chu, Chishih; Huang, Pei-Yu; Chen, Hung-Ming; Wang, Ying-Hsiang; Tsai, I-An; Lu, Chih-Cheng; Chen, Che-Chun

doi:10.1186/s12866-016-0794-4

BMC Microbiology

Table 5 Death and symptom number of tilapia response to seven serotype Ia fish isolates and seven serotype Ia and III human isolates

From: Genetic and pathogenic difference between Streptococcus agalactiae serotype Ia fish and human isolates

Bacterial Source	Serotype	Strain	Repeat^a	Symptom									Mortality
				Hemorrhage of pectoral fin			Hemorrhage of gill			Viscous black and common ascites			I^a			II^b
				No.	Mean		No.	Mean		No.	Mean (%)		No.	Mean		Bacterial dose (1 × 10⁸ cfu)	Mean (%)
				No.	No.	%	No.	No.	%	No.			No.	No.	%	Bacterial dose (1 × 10⁸ cfu)	Mean (%)
Fish isolates	Control		1	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0	16.7 ± 0
	Control		2	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0	16.7 ± 0
	Ia	886	1	0/6	0.5/6	8.3	2/6	2.5/6	41.7	5/6	4/6	66.7	6/6	6/6	100	3.1–3.8	100 ± 0
		886	2	1/6	0.5/6	8.3	3/6	2.5/6	41.7	3/6	4/6	66.7	6/6	6/6	100	3.1–3.8	100 ± 0
		900	1	2/6	3/6	50	3/6	3.5/6	58.3	5/6	5.5/6	91.7	6/6	6/6	100	2.5–2.6	100 ± 0
		900	2	4/6	3/6	50	4/6	3.5/6	58.3	6/6	5.5/6	91.7	6/6	6/6	100	2.5–2.6	100 ± 0
		912	1	0/6	0.5/6	8.3	0/6	0.5/6	8.3	2/6	1.5/6	25	5/6	3.5/6	58.3	2.1–3.1	54.2 ± 25.0
		912	2	1/6	0.5/6	8.3	1/6	0.5/6	8.3	1/6	1.5/6	25	2/6	3.5/6	58.3	2.1–3.1	54.2 ± 25.0
		948	1	3/6	2.5/6	41.7	3/6	2.5/6	41.7	4/6	4/6	66.7	6/6	5.5/6	91.7	3.1–3.8	91.7 ± 16.7
		948	2	2/6	2.5/6	41.7	2/6	2.5/6	41.7	4/6	4/6	66.7	5/6	5.5/6	91.7	3.1–3.8	91.7 ± 16.7
		953-1	1	0/6	1/6	16.7	2/6	1.5/6	25	2/6	1.5/6	25	6/6	6/6	100	2.5–2.6	100 ± 0
		953-1	2	2/6	1/6	16.7	1/6	1.5/6	25	1/6	1.5/6	25	6/6	6/6	100	2.5–2.6	100 ± 0
		954	1	0/6	1/6	16.7	3/6	3/6	50	6/6	4.5/6	75	6/6	5/6	83.3	2.1–3.1	54.2 ± 25.0
		954	2	2/6	1/6	16.7	3/6	3/6	50	3/6	4.5/6	75	4/6	5/6	83.3	2.1–3.1	54.2 ± 25.0
		1004	1	1/6	0.5/6	8.3	1/6	1/6	16.7	5/6	4/6	0	6/6	5/6	83.3	2.1–3.4	95.8 ± 8.3
		1004	2	0/6	0.5/6	8.3	1/6	1/6	16.7	3/6	4/6	0	4/6	5/6	83.3	2.1–3.4	95.8 ± 8.3
Human Isolate	Control		1	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	1/6	1/6	16.7	0	16.7 ± 0
	Control		2	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	1/6	1/6	16.7	0	16.7 ± 0
	Ia	G91	1	0/6	0/6	0	0/6	0/6	0	1/6	0.5/6	8.3	1/6	0.5/6	8.3	2.3–2.9	8.3 ± 9.62
		G91	2	0/6	0/6	0	0/6	0/6	0	0/6	0.5/6	8.3	0/6	0.5/6	8.3	2.3–2.9	8.3 ± 9.62
		G108	1	0/6	0/6	0	0/6	0/6	0	1/6	0.5/6	8.3	0/6	0.5/6	8.3	1.8–2.2	16.7 ± 13.6
		G108	2	0/6	0/6	0	0/6	0/6	0	0/6	0.5/6	8.3	1/6	0.5/6	8.3	1.8–2.2	16.7 ± 13.6
		G110	1	0/6	0/6	0	0/6	0/6	0	0/6	0.5/6	8.3	1/6	1/6	16.7	2.1–3.0	8.3 ± 8.34
		G110	2	0/6	0/6	0	0/6	0/6	0	1/6	0.5/6	8.3	1/6	1/6	16.7	2.1–3.0	8.3 ± 8.34
	III	G116	1	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	2/6	1/6	16.7	1.9–2.6	29.2 ± 21.0
		G116	2	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0/6	1/6	16.7	1.9–2.6	29.2 ± 21.0
		G1	1	1/6	0.5/6	8.3	1/6	0.5/6	8.3	0/6	0/6	0	1/6	0.5/6	16.7	1.3–2.0	16.7 ± 13.6
		G1	2	0/6	0.5/6	8.3	0/6	0.5/6	8.3	0/6	0/6	0	0	0.5/6	16.7	1.3–2.0	16.7 ± 13.6
		G78	1	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	0	0/6	16.7	1.4–1.9	12.5 ± 16.0
		G78	2	0/6	0/6	0	0/6	0/6	0	0/6	0/6	0	1	0/6	16.7	1.4–1.9	12.5 ± 16.0
		G102	1	0	0/6	0	0/6	0/6	0	0/6	0/6	0	0	0.5/6	16.7	1.3–2.1	12.5 ± 16.0
		G102	2	0	0/6	0	0/6	0/6	0	0/6	0/6	0	0	0.5/6	16.7	1.3–2.1	12.5 ± 16.0

^a6 fish were used in each repeat
^bSix fish were used for each isolate and each data were average of four repeats

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