Fig. 3

B. abortus infection modulates the expression of A20 and IkBα in a miR-125b-5p-dependent manner. a RAW264.7 cells were infected with B. abortus for different periods of time. b RAW264.7 cells were transfected with either Scr-miR or Pre-miR-125b-5p and infected with B. abortus for 4 or 8 h. Expression of A20 in a and b were determined by immunoblotting with A20 antibody. c RAW264.7 cells transfected with control anti-oligonucleotides (anti-oligo (Con)) or anti-miR-125b-5p oligonucleotides (anti- miR-125b-5p-oligo) were infected with B. abortus and their RNA was subjected to qRT-PCR analysis of miR-125b-5p (top panel). A20 expression in cell lysates was measured by immunoblotting with A20 antibody. d RAW264.7 cells transfected with Scr-miR or Pre-miR-125b-5p were infected with B. abortus for the indicated time periods, and IkBα expression was monitored in RAW264.7 cell lysates with IkBα antibody. The blot is representative of 3 independent experiments. Quantitative data for A20 and IkBα expression are shown under the Western blotting data. Data are expressed as mean ± SEM (n = 3). The maximal ratio of IkBα or A20 expression /β-actin in the group was defined as 100 % (or control). *P < 0.05 VS control. NS indicates no significance