Table 1 Primers used for drug resistant gene amplification
From: Genotypic characterization of drug resistant Mycobacterium tuberculosis in Quebec, 2002-2012
Genes | Primers (Sequences 5’ → 3’) | Product size (bp) |
|---|---|---|
katG | katG-F (5’-GACATTCGCGAGACGTTTCGG-3’) | 642 |
katG-R (5’GCTCTTAAGGCTGGCAATCTCG-3’) | ||
inhA | inhA-F (5’-CTATATCTCCGGTGCGGTCA-3’) | 469 |
inhA-R (5’-CTTGGCCATCGAAGCATAC-3’) | ||
inhA-Prom | inhA-Prom-1 (5’-TCCGTCATGGTCGAAGTGTG-3’) | 211 |
inhA-Prom-2 (5’-GGTAACCAGGACTGAACGGG-3’) | ||
rpoB | rpoB-F (5’-ACGGTCGGCGAGCTGATCC-3’) | 351 |
rpoB-R (5’-CAGACCGATGTTGGGCCCCT-3’) | ||
pncA | pncA-F1 (5’-GGCGTCATGGACCCTATATC-3’) | 670 |
pncA-R1 (5’-CAACAGTTCATCCCGGTTC-3’) | ||
embB | embB-1 (5’- TGATATTCGGCTTCCTGCTC-3’) | 417 |
embB-2 (5’- ACCGCTCGATCAGCACATAG-3’) | ||
∆katG a | katGD-1 (5’-CCGGTCAAGAAGAAGTACGG-3’) | 590 |
katGD-2 (5’-CTCTTCGTCAGCTCCCACTC-3’) | ||
katGD-5 (5’-GGGGAACATCAAAGTGTCCT-3’) | 400 | |
katGD-6 (5’-GATACCCATGTCGAGCAGGT-3’) |