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Fig. 4 | BMC Microbiology

Fig. 4

From: Pet serine protease from enteroaggregative Escherichia coli stimulates the inflammatory response activating human macrophages

Fig. 4

Immunofluorescence analysis of NF-kB activation. a Human macrophages were stimulated during 30 min with Pet (10 μg/ml) or LPS (Positive control) and non-stimulated. The nuclear translocation is determined (arrows) by the blue fluorescence (Hoechst staining) and the NF-kB p65 subunit (arrows) by its green fluorescence (Dylight 488). b Percentage of nucleic positive cells to p65. The results are representative of three independent experiments

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