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Fig. 5 | BMC Microbiology

Fig. 5

From: Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrIC gene cluster via a bacteriophage

Fig. 5

Southern Blot of Eco32I-digested chromosomal DNA. i. Agarose gel of digested genomic DNA. ii. Southern blot analysis of digested genomic DNA. a Bangladeshi, and Japanese serotypes 1a and 1b strains, with a gtrIC probe. Lane 1. Marker SPP-I/EcoRI; 2. SFL1613 (control strain); 3. SFL1287 (1a-Jpn); 4. SFL1288 (1a-Jpn); 5. SFL1492 (1a-Bangladesh); 6.SFL1493(1a-Bangladesh); 7. SFL1494(1a-Bangladesh); 8.SFL1495(1a-Bangladesh); 9. SFL1496(1b-Bangladesh); 10.SFL1497(1b-Bangladesh); 11.SFL1498(1b-Bangladesh); 12. SFL1499(1b-Bangladesh). b UK and Japanese serotype 1b strains, with a gtrIC probe. Lane 1. Marker SPP-I/EcoRI; 2. SFL1613 (control strain); 3. B1118 (control negative strain); 4. SFL1417 (1b-NCTC-UK); 5. SFL276 (1b-Japan); 6.SFL1289(1b-Japan); 7. SFL1300(1b-Japan); 8.SFL1309(1b-Japan); 9. SFL1315(1b-Japan); 10.SFL1316(1b-Japan); 11.SFL1277(1b-rough-Japan); 12. SFL1278(1b-rough-Japan). c Ten selected UK, Bangladeshi and Japanese serotype 1b strains, with a gtrIC cluster probe. Lane 1. SPP-I/EcoRI; 2. SFL1613 (control strain); 3. SFL1287(1a-Japan); 4. SFL1300 (1b-NCTC-UK); 5. SFL1315 (1b-Japan); 6.SFL1316(1b-Japan); 7. SFL1499(1b-B); 8.SFL1498(1b-Rough); 9. SFL1497(1b-B); 10.SFL1496(1b-B); 11.SFL1417(1b-UK); 12. SFL1277(1b-rough-Japan)

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