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Table 1 Bacterial strains and plasmids used in this study

From: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity

Strains or plasmids Description Source
 pQK663 Asd+ vector, p15A ori, specr Derived from pYA3332 [26]
 pQK163 Insertion of crp into pQK663 This work
 pET-32a-crp For the expression of P. multocida Crp This work
 pMC-Express A broad host-range shuttle vector derived from pMIDG100, chloramphenicolr [27]
 pYA4278 pRE112 derivative, sacB mobRP4 R6K ori Cm+ [29]
 pQK174 pYA4278-Δcrp This work
 pQK175 pYA4278-Δcrp::kan, for deletion of crp in P. multocida 0818 This work
 pQK176 Insertion of crp into pMC-Express This work
 S184 S. Typhimurium Δasd66 Lab collection
 S411 S. Typhimurium Δasd66 Δcrp89 Lab collection
P. multocida 0818 Wild-type and virulent. Capsular serotype A:1. Lab collection
 S416 P. multocida 0818 Δcrp::kanR This work
 χ7232 E.coli K-12, endA1 hsdR17 (rK-, mK +) glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR80dlac Δ(lacZ)M15) [30]
 χ7213 E.coli K-12, thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpirasdA4zhf-2::Tn10 [30]