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Table 1 Bacterial strains and plasmids used in this study

From: Identification of the crp gene in avian Pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity

Strains or plasmids Description Source
Plasmids
 pQK663 Asd+ vector, p15A ori, specr Derived from pYA3332 [26]
 pQK163 Insertion of crp into pQK663 This work
 pET-32a-crp For the expression of P. multocida Crp This work
 pMC-Express A broad host-range shuttle vector derived from pMIDG100, chloramphenicolr [27]
 pYA4278 pRE112 derivative, sacB mobRP4 R6K ori Cm+ [29]
 pQK174 pYA4278-Δcrp This work
 pQK175 pYA4278-Δcrp::kan, for deletion of crp in P. multocida 0818 This work
 pQK176 Insertion of crp into pMC-Express This work
Strains
 S184 S. Typhimurium Δasd66 Lab collection
 S411 S. Typhimurium Δasd66 Δcrp89 Lab collection
P. multocida 0818 Wild-type and virulent. Capsular serotype A:1. Lab collection
 S416 P. multocida 0818 Δcrp::kanR This work
 χ7232 E.coli K-12, endA1 hsdR17 (rK-, mK +) glnV44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 λpir deoR80dlac Δ(lacZ)M15) [30]
 χ7213 E.coli K-12, thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpirasdA4zhf-2::Tn10 [30]