Fig. 3
Analysis of T. hirsuta 072 secretome. The extracellular proteins were extracted from cultural broth and resolved by 2D electrophoresis (see Methods). Proteins secreted in GP medium (submerged growth) without induction (left) and induced by CuSO4 (right), respectively are shown on 5th day of cultivation (panel a) and on 8th day of cultivation (panel b). The extracellular proteins expressed in LC medium (surface growth) are displayed for 3th day of cultivation without and with added CuSO4 (panel c). To observe differential expression, for each panel, equal amounts of total protein were loaded on both gels. Proteins identified by MALDI-TOF/TOF MS analysis are marked on the gels: Lac – laccase; MnP – manganese peroxidase; VP – versatile peroxidase; GLOX – copper radical oxidase (glyoxal oxidase); PG1 -Endo-polygalacturonase PG1, GH2 – glycoside hydrolase family 2; GH13 – glycoside hydrolase family 13; GH51 – glycoside hydrolase family 51; GH92 – glycoside hydrolase family 92; CBH – cellobiohydrolase I; G2 – glucoamylase G2; CE – carbohydrate esterase family 16; S53 - family S53 protease; Pept - aspartic peptidase; CP - cerato-platanins