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Table 2 oprD genotypes among 78 bacteremic IRPA isolates

From: Overproduction of active efflux pump and variations of OprD dominate in imipenem-resistant Pseudomonas aeruginosa isolated from patients with bloodstream infections in Taiwan

Major types of mutationa No. of isolate Description
Amino acid frameshift produced by 1- and 2-bp insertion or deletion 10 Deletion of: 1 bp (G) at nt 311; 2 bp (TG) at nt 407-408; 1 bp (C) at nt 456 (2 isolates)
Insertion of: 1 bp (G) at nt 555; 1 bp (T) at nt 977; 2 bp (AC) at nt 1050-1051 (2 isolates); 1 bp (C) at nt 1205 (2 isolates)
Amino acid frameshift produced by partial deletion 5 11-bp deletion beginning at nt 140, nt 175, nt 473
13-bp deletion beginning at nt 175, nt 208
Premature stop codon (point mutation) 14 CAG → TAG at nt 55-57, nt 472-474, nt 883-885, nt 1270-1272 (3 isolates)
TGG → TAG at nt 193-195
TGG → TAG at nt 829-831 (3 isolates)
TGG → TGA at nt 829-831
TGG → TAG at nt 1015-1017 (2 isolates)
TAT → TAG at nt 1048-1050
Interruption by IS 10 Transposase at nt 37, 50 (2 isolates), 271, 434, 562, 571, 623, 652, 694
Amino acid substitution 19 Polymorphisms: D43N, S57E, S59R, T103S, K115T, V127L, F170L, E185Q, P186G, V189T, E202Q, I210A, E230K, D231N, S240T, L246V, D249E, L252V, N262T, R310E, A315G, S403A, Q424E, and G425A
Shortening of putative loop L7 18 Putative loop L7 (372 V-DSSSSYAGL-Y384)
Identical 2 -
  1. aOnly 2 isolates (926 and 5718) showed identical sequences of oprD to PAO1. The other 76 isolates contained amino acid variation compared to the sequence of oprD in PAO1