Fig. 1

Phylogenetic tree of G. duodenalis based on nucleotide sequences of the tpi gene (a) and gdh gene (b). Trees were constructed using the neighbor-joining method based on genetic distance calculated by the Kimura 2-parameter model, implemented in MEGA version 5.2. Bootstrap values > 50 % from 1,000 replicates is shown on nodes. Reference sequences from the major G. duodenalis sub-assemblages are written by blue color in bold. Isolates showed known and novel sequences obtained from this study are marked by rectangles (blue) and triangles (red), respectively