Persistence of bacterial indicators and zoonotic pathogens in contaminated cattle wastes

BMC Microbiology

Table 3 Results of real-time PCR (DHI, DH2 and DH3) for MAP detection

Tests performed in three independent PCR reactions on DNA extracted from seven samples (A, B1, B3, C, D, E, F) obtained by joining the corresponding five sampling units
^aPrimers described by Herthnek and Bölske (2006)
^bPCR positivity (+) was confirmed by the sequencing of at least one PCR product per reaction replicates. Percent of similarity with reference MAP sequences in NCBI Genebank was at least 98 %

ISSN: 1471-2180