|
Escherichia coli strain
|
|
DH5α
|
F- 80dlacZ, ΔM15, Δ(lacZYA-argF)U169, endA1, recA1, hsdR17(rK–mK+), deoR, thi-1, supE44, gyrA96, relA1 λ-
|
Invitrogen, CA, USA
|
|
Yeast strain
|
|
Saccharomyces cerevisiae AH109
|
MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2 : : GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3 : : MEL1UAS-MEL1TATA-lacZ
|
[61]
|
|
Erwinia amylovora strains
|
|
Ea1189
|
Wild type
|
[62]
|
|
Ea1189∆hrcU
|
hrcU deletion mutant, CmR
|
This study
|
|
Ea1189∆hrpP
|
hrpP deletion mutant, CmR
|
This study
|
|
Plasmids
| | |
|
pBBR1-MCS3
|
TcR, broad host-range cloning vector
|
[54]
|
|
pGADT7
|
LEU2, AmpR, Y2H activation vector
|
Clontech, CA, USA
|
|
pGBKT7
|
TRP1, KmR, Y2H bait vector
|
Clontech, CA, USA
|
|
pLRT201
|
AmpR, pMJH20 expressing DspE(1-737)-CyaA
|
[38]
|
|
pMJH20
|
AmpR, pWSK29 containing codons 2 to 406 of CyaA
|
[63]
|
|
pRRM1
|
TcR, pBBR1-MCS3 containing hrcU
|
This study
|
|
pRRM2
|
TcR, pBBR1-MCS3 containing hrcU
N266A
|
This study
|
|
pRRM3
|
AmpR, pGADT7 containing hrpP
|
This study
|
|
pRRM4
|
AmpR, pGADT7 containing hrpJ
|
This study
|
|
pRRM5
|
KmR, pGBKT7 containing hrcU
|
This study
|
|
pRRM6
|
KmR, pGBKT7 containing hrcU
N266A
|
This study
|
|
pRRM7
|
KmR, pGBKT7 containing hrcU
209-360
|
This study
|
|
pRRM8
|
KmR, pGBKT7 containing hrcU
209-360, N266A
|
This study
|
|
pRRM9
|
TcR, pAlter-Ex1 containing hrcU
|
This study
|
|
pRRM10
|
AmpR, pAlter-Ex1 containing hrcU
N266A
|
This study
|
|
pAlter-Ex1
|
TcR, mutagenesis vector
|
Promega, WI, USA
|
|
pKD3
|
AmpR, CmR mutagenesis cassette template
|
[59]
|
|
pKD46
|
AmpR, expresses λ red recombinase
|
[59]
|
