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Table 2 Bacterial strains and plasmids and their relevant characteristics

From: HrcU and HrpP are pathogenicity factors in the fire blight pathogen Erwinia amylovora required for the type III secretion of DspA/E

Strains & Plasmids Relevant characteristicsa Source or reference
Escherichia coli strain
 DH5α F- 80dlacZ, ΔM15, Δ(lacZYA-argF)U169, endA1, recA1, hsdR17(rK–mK+), deoR, thi-1, supE44, gyrA96, relA1 λ- Invitrogen, CA, USA
Yeast strain
Saccharomyces cerevisiae AH109 MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2 : : GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, URA3 : : MEL1UAS-MEL1TATA-lacZ [61]
Erwinia amylovora strains
 Ea1189 Wild type [62]
 Ea1189∆hrcU hrcU deletion mutant, CmR This study
 Ea1189∆hrpP hrpP deletion mutant, CmR This study
Plasmids   
 pBBR1-MCS3 TcR, broad host-range cloning vector [54]
 pGADT7 LEU2, AmpR, Y2H activation vector Clontech, CA, USA
 pGBKT7 TRP1, KmR, Y2H bait vector Clontech, CA, USA
 pLRT201 AmpR, pMJH20 expressing DspE(1-737)-CyaA [38]
 pMJH20 AmpR, pWSK29 containing codons 2 to 406 of CyaA [63]
 pRRM1 TcR, pBBR1-MCS3 containing hrcU This study
 pRRM2 TcR, pBBR1-MCS3 containing hrcU N266A This study
 pRRM3 AmpR, pGADT7 containing hrpP This study
 pRRM4 AmpR, pGADT7 containing hrpJ This study
 pRRM5 KmR, pGBKT7 containing hrcU This study
 pRRM6 KmR, pGBKT7 containing hrcU N266A This study
 pRRM7 KmR, pGBKT7 containing hrcU 209-360 This study
 pRRM8 KmR, pGBKT7 containing hrcU 209-360, N266A This study
 pRRM9 TcR, pAlter-Ex1 containing hrcU This study
 pRRM10 AmpR, pAlter-Ex1 containing hrcU N266A This study
 pAlter-Ex1 TcR, mutagenesis vector Promega, WI, USA
 pKD3 AmpR, CmR mutagenesis cassette template [59]
 pKD46 AmpR, expresses λ red recombinase [59]
  1. aCmR, TcR, AmpR, KmR indicates resistance to chloramphenicol, oxytetracycline, ampicillin and kanamycin