Table 2 Bacterial strains and plasmids and their relevant characteristics

 DH5α

F- 80dlacZ, ΔM15, Δ(lacZYA-argF)U169, endA1, recA1, hsdR17(rK–mK+), deoR, thi-1, supE44, gyrA96, relA1 λ-

Invitrogen, CA, USA

  Saccharomyces cerevisiae AH109

MATa, trp1-901, leu2-3, 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2 : : GAL1_UAS-GAL1_TATA-HIS3, GAL2_UAS-GAL2_TATA-ADE2, URA3 : : MEL1_UAS-MEL1_TATA-lacZ

[61]

 Ea1189

Wild type

[62]

 Ea1189∆hrcU

hrcU deletion mutant, Cm^R

This study

 Ea1189∆hrpP

hrpP deletion mutant, Cm^R

This study

Plasmids

 pBBR1-MCS3

Tc^R, broad host-range cloning vector

[54]

 pGADT7

LEU2, Amp^R, Y2H activation vector

Clontech, CA, USA

 pGBKT7

TRP1, Km^R, Y2H bait vector

Clontech, CA, USA

 pLRT201

Amp^R, pMJH20 expressing DspE(1-737)-CyaA

[38]

 pMJH20

Amp^R, pWSK29 containing codons 2 to 406 of CyaA

[63]

 pRRM1

Tc^R, pBBR1-MCS3 containing hrcU

This study

 pRRM2

Tc^R, pBBR1-MCS3 containing hrcU _N266A

This study

 pRRM3

Amp^R, pGADT7 containing hrpP

This study

 pRRM4

Amp^R, pGADT7 containing hrpJ

This study

 pRRM5

Km^R, pGBKT7 containing hrcU

This study

 pRRM6

Km^R, pGBKT7 containing hrcU _N266A

This study

 pRRM7

Km^R, pGBKT7 containing hrcU _209-360

This study

 pRRM8

Km^R, pGBKT7 containing hrcU _{209-360, N266A}

This study

 pRRM9

Tc^R, pAlter-Ex1 containing hrcU

This study

 pRRM10

Amp^R, pAlter-Ex1 containing hrcU _N266A

This study

 pAlter-Ex1

Tc^R, mutagenesis vector

Promega, WI, USA

 pKD3

Amp^R, Cm^R mutagenesis cassette template

[59]

 pKD46

Amp^R, expresses λ red recombinase

[59]