Fig. 2

Phenotypic characterization of HrcU-related mutant strains in Ea1189. WT Ea1189, Ea1189∆hrcU and Ea1189∆hrcU stains expressing native HrcU from pRRM1 or HrcU_N266A from pRRM2 were inoculated into a immature pear fruits and b Nicotiana benthamiana. Pear fruit necrosis was recorded 6 days post inoculation while the hypersensistive response in N. benthamiana was observed 16 h post inoculation. Ea1189∆hrcU was non-pathogenic and unable to elicit a hypersensitive response while pRRM2 was unable to complement the hrcU null mutation