Table 3 Lists of primers used in this study
Primers name (Target) | Restriction site (Use) | Sequence (5’–3’) | Source |
|---|---|---|---|
ZE565 (pefC) | EcoR1 | GGGGAATTCTTATGGTGGGTCATTGTTG | This study |
ZE566 (pefC) | EcoR1 | AATGAATTCTAAGCGAGGGATGAGCTGTG | This study |
ZE634 (pefRCD) | SpeI | AAGGACTAGTGGTCTTGGCTAATAAGGCG | This study |
ZE635 (pefRCD) | SpeI | AGGGACTAGTTTGGGATTCATGTTAGCGAG | This study |
ZE553 (pefR) | NA | TGAGGAGACAACGATGCTTAAAGAC | This study |
ZE554 (pefC) | ClaI | AAAAAATCGATCAGGGTTGTTTGACTTAG | This study |
ZE458 (pefR) | NA | CACAAGTGATAGGTGATTTACGTG | This study |
ZE562 (pefR) | NA | CCTTGAGGACCTGCTAGATGCTCTAC | This study |
ZE649 (pefD) | NA | GGAGAATTGCAGCTGGCC | This study |
ZE650 (pefD) | NA | AGCTGGTGAACGCTGGTGC | This study |
ZE653 (pefC) | NA | GCGTTCAAGAAGGCCTTAAGTC | This study |
ZE654 (pefC) | NA | CCACCAACTCTGCGTGTGTTC | This study |
ZE581 (rpsL) | NA | CAGATTCACCAGCTTTGAAC | [15] |
ZE582 (rpsL) | NA | CAACACGAGTAGCAACG | [15] |
SpecFw | NA | GTGAGGAGGATATATTTGAATACATACGAA | This study |
SpecRev | NA | GTCCATTCAATATTCTCTCCAAGATAACTA | This study |