Table 1 Lists of bacterial strains and plasmids used in this study
Strain name | Characteristics | References |
|---|---|---|
S. pyogenes | ||
 NZ131 | M49 serotype isolated from acute post-glomerulonephritis infection | [57] |
 ZE4951 | NZ131 derivative with pefC::pMZ1 mutation | This study |
 ZE4951/pANKITA5b | ZEM4951 strain complemented with pefRCD locus expressed from plasmid pANKITA5b | This study |
 ZE4951/pKSM201 | ZEM4951 strain harboring the pKSM201 vector | This study |
E. coli | ||
 DH5α | hsdR17 ecA gyrA endA1 relA1 | [58] |
Plasmids | ||
 pMZ1 | pUC-Spec derivative containing pefC internal fragment and the spec resistance gene aad9 | This study |
 pKSM201 | Shuttle vector containing the kanamycin resistance gene aphA3 | [56] |
 pANKITA5b | pKSM201 derivative carrying the pefRCD genes | This study |