Fig. 6

Uptake of B. abortus induces trafficking of PAFR to endosomes. (a) RAW 264.7 cells were pretreated for 5 min with PAF (200 nM), and then B. abortus in medium containing 1 mg/ml of TRDx was deposited onto cells followed by incubation at 37 °C for 30 min. Immunostaining with FITC-labeled PAFR (green) was used to detect the colocalization of PAFR and endosomes (red). The merged images showed colocalization of PAFR and endosomes (arrow). (b) The quantitative analysis results of experiment in (a). The colocalization number of endosomes with PAFR was counted, and the data are presented as a percentage per 100 cells. All results are representatives of three separate experiments (** P < 0.01). All scale bars in images represent 10 μm