Table 5 Oligonucleotide primers used in this study
Primer | Resultant construct | Sequence (5′-3′) |
|---|---|---|
BamINLB | pBYM3, pBYM4 and pBINU1 | CGGGATCCTCAATTTGTTTACACCAC |
BamINRB | pBYM3, pBYM4 and pBINU1 | CGGGATCCCAGTTTAAACTATCAGTG |
SRS2Fw | pSRS2 | GGAATTCCAGGAGTGAAGACATCTGC |
SRS2Rv | pSRS2 | GGAATTCCGACTTGGGACTATTGGAC |
RAD52Fw | pRAD52 | GGAATTCTAACGGTGAGTGTGGCAACG |
RAD52Rv | pRAD52 | GGAATTCTGAACCTAAGGATTCCGCTG |
SMI1Fw | pSMI1 | GGAATTCCACTGATCCATTTACCTGC |
SMI1Rv | pSMI1 | GGAATTCCTCATGCTCTTCAATGTCG |
ERG28_2_Fw | pERG28 | CGAGGAATTCTCGGGGACAACAACTTCAG |
ERG28Rv | pERG28 | GGAATTCTGAGCTGGAGCAGACATTG |
URA3-BamHIfw | pBINU1and pBINU2 | CGGGATCCCGAGTCGCATAAGGGAGAGC |
URA3-BamHIrv | pBINU1 | CGGGATCCCGATCAGCGTGGTCGTGAAG |
URA3-EcoRI | pBINU2 | CGGAATTCCTGAAGCTCTAATTTGTG |
URA3-probe-Fw | URA3 probe | CTTAACCCAACTGCACAGAACA |
URA3-probe-Rv | URA3 probe | GCAATAAAGCCGATAACA |
Amp-probe-Fw | Amp r gene probe | TGCAATGATACCGCGAGAC |
Amp-probe-Rv | Amp r gene probe | CGAACTGGATCTCAACAGCGGTAA |
pBY1-RB-Seq | for sequencing | GAACGCGCAATAATGGTTTCTG |
pBY1-RB-Seq2 | for sequencing | GGGCGCACCGCAGATGGAAA |