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Table 1 Yeast mutant strains exhibiting low AMT efficiency

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Yeast strain (genotype) AMT efficiencya AMT efficiency of complemented strainb TKC efficiencyc Growth of yeast cells (fold)d Growth ratio
  % of wild type ± SD % of wild type ± SD % of wild type ± SD (A) with donor cells (B) w/o donor cells (A/B)
wild type (100) (100) (100) 3.5 ± 1.7 11.8 ± 4.1 0.30
srs2Δ 5.6 ± 2.8** 103.2 ± 21.7 102.2 ± 22.2 5.3 ± 2.2 15.3 ± 6.9 0.34
rad52Δ 4.8 ± 1.9** 187.9 ± 41.1* 52.5 ± 27.2* 4.7 ± 0.2 6.1 ± 0.6 0.77
smi1Δ 5.0 ± 2.4** 87.8 ± 5.1* 49.8 ± 27.2* 4.8 ± 2.2 10.0 ± 3.8 0.48
erg28Δ 24.5 ± 9.5** 123.3 ± 70.4 25.1 ± 18.6** 8.4 ± 1.5 8.2 ± 1.3 1.02
  1. a Yeast strains were cocultivated with Agrobacterium strain EHA105 (pBY1). The AMT efficiency of the wild type yeast strain was (1.9 ± 0.1) × 10-3
  2. b Each mutant strain was introduced a corresponding wild type gene cloned in centromeric vector pRS313 (see Table 4). The AMT efficiency of the wild type strain harboring the pRS313 vector was (4.1 ± 1.7) × 10-3
  3. c Yeast strains were cocultivated with E. coli strain HB101 (pRH210, pAY205) for trans-kingdom conjugation ( TKC). The TKC efficiency of the wild type yeast strain was (1.2 ± 0.3) × 10-3
  4. d Fold increase of recipient cell number after co-cultivation (A) with or (B) without donor cells. Each value is the average of three experiments. SD = standard deviations
  5. Differences were statistically significant compared to the wild type strain by Student’s t-test. *p <0.05, **p <0.01