Skip to main content
Fig. 7 | BMC Microbiology

Fig. 7

From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA

Fig. 7

Southern blot analysis of yeast AMT transformants. AMT yeast transformants were obtained by co-cultivation with the donor bacterial strain EHA105 (pBY1). a Schematic diagram of the reference plasmids pBY1 and pYAC4-B, and the T-DNA region of pBY1. Tel, Tetrahymena telomere sequences. b Total DNA extracted AMT transformants of the wild-type yeast (W1–W6), c rad52Δ (R1–R6) and d srs2Δ (S1–S6). EcoRI-digested (E) DNA or undigested (-) DNA were used for Southern blot analysis probed with a mixture of URA3 probe and Amp r genes (see location of each probe in panel a). Control DNAs were pYAC4-B (-), and pYAC4-B digested with BamHI (B) and with BamHI and EcoRI (BE)

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us