Fig. 1From: DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNAEffect of input recipient cell number on AMT efficiency. Yeast mutant strains were co-cultivated with Agrobacterium EHA105 harboring pBY1. The number of input yeast cells ranged from 2.5 × 105 to 4 × 106 cells. Relative efficiency was calculated by dividing AMT efficiency by that of yeast wild type strain. Error bars indicated the standard deviations of triplicate assays. Differences were statistically significant compared to the wild type strain to mutant strains by Student’s t-test. ** p <0.01Back to article page