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Fig. 5 | BMC Microbiology

Fig. 5

From: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica

Fig. 5

White blood cell (WBC) migration and cytokine secretion by macrophage (6 %) / hepatocyte (94 %) cultures co-incubated with 2 x105 WBC in a transwell filter insert. a Percentage of detected CD68b positive granulocytes by flow cytometry 24, 48 and 72 h post infection. Cytokine secretion of b TNFα, c IL-1β, d IL-6 and e fractalkine (FKN) in response to infection with LVS (open bars), spp. holarctica (grey filled bars) or spp. mediasiatica (black filled bars) or untreated control (hatched bars) after 24, 48 and 72 h of cell culture. Statistical significance was calculated using student’s t-test: * p < 0.05, *** p < 0.001 compared to identical time point after infection with LVS; # p < 0,05, ### p < 0.001 compared to identical time point after infection with spp. holarctica

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