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Fig. 3 | BMC Microbiology

Fig. 3

From: A human macrophage – hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica

Fig. 3

a Immunofluorescence staining of macrophages marker protein CD68 (green) and apoptosis marker cleaved caspase-3 (cl. casp-3, red) in co-culture of 6 % macrophages and 94 % hepatocytes. Nuclei are stained with DAPI (blue). Cells were infected with LVS, spp. holarctica and spp. mediasiatica and subsequently co-cultured for 24, 48 and 72 h, respectively. b-d Quantification of the mean fluorescence intensity (MFI) of labeled antibody for detection of the apoptosis marker cleaved caspase-3 (cCasp-3) in macrophages b) 24h, c) 48h and d) 72h post infection. Whisker dot plots show boxes ranging from 10th (lower box boundary) to 90th (upper box boundary). Vertical line inside the box marks the median. Outliers are shown as filled circles. Statistical significance was calculated using one-way ANOVA with Dunnett's multiple comparisons (* p < 0.05 for indicated conditions)

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