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Table 1 Quantification of biofilm formation by measuring optical density at 580 nm (OD580) of crystal violet dye attached to the cells forming biofilms on glass tubes at 27 °C under static conditions at 60 h

From: Contributions of tropodithietic acid and biofilm formation to the probiotic activity of Phaeobacter inhibens

Strains

OD580 (±SD)a

P. inhibens S4Sm

3.89 ± 0.06

P. inhibens WZ10 (clpX-)

3.90 ± 0.12

P. inhibens WZ11 (clpX+)

4.0 ± 0.06

P. inhibens WZ20 (exoP-)

1.60 ± 0.09b

P. inhibens WZ21 (exoP+)

3.90 ± 0.10

V. anguillarum NB10Sm

0.58 ± 0.02b

V. tubiashii RE22Sm

0.54 ± 0.02b

R. crassostreae CV919Sm

0.52 ± 0.08b

  1. aBiofilm formation quantified by crystal violet dye assay as described in the Materials and Methods. The data presented are the average of two independent experiments, each with three replicates. SD = standard deviation
  2. bStatistically significant difference (P <0.05) compared to S4Sm