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Fig. 3 | BMC Microbiology

Fig. 3

From: Flagellin and GroEL mediates in vitro binding of an atypical enteropathogenic Escherichia coli to cellular fibronectin

Fig. 3

Immunofluorescence assay. Bacterial pellets from aEPEC BA2103 (panels a and d), aEPEC 2271-1/85 (panels b and e) and K12 DH5α (panels c and f) were fixed with 4 % paraformaldehyde on slide glasses and incubated with rabbit anti-H11 (panels a to c) or with rabbit anti-GroEL (panels d to f) followed by an incubation with goat anti-rabbit IgG conjugated to FITC. The immunoassay with intact, non-permeabilized bacteria was visualized on Axioskop fluorescence microscope with a 400X magnification

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BMC Microbiology

ISSN: 1471-2180

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