Fig. 1

Colony and cellular morphology of the C. difficile mfd mutant. a Liquid cultures (1 μl) of strains 630Δerm and the mfd mutant containing plasmid vector alone (pMTL960) or with the mfd gene (pSEW070) as appropriate were spotted on to TY or TYG agar and grown for 3 days. Representative colonies were photographed. Bar = 1 cm. b Cultures of parental strain 630Δerm and the mfd mutant containing pMTL960 vector or pSEW070 were grown in TY or TYG broth and photographed under phase contrast microscopy. Bar = 5 μm