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Table 3 Identification of Gram-negative isolates using biochemical methods

From: Microbiological testing of pharmaceuticals and cosmetics in Egypt

Isolate code

Identification based on conventional biochemical tests

Identification based on API 20E kit

Final conclusion

Result (%ID)

Level of discrimination

1

Erwinia cacticida or Erwinia dissolvens

Ent. cloacae (94.3 %)

Good

Contradiction (Enterobacter/Erwinia spp.)a

8A

Providencia alcalifaciens, Providencia rustigianii or Providencia stuartii

Providencia alcalifaciens/rustigianii (72 %)

Very good identification to the genus

Providencia alcalifaciens/rustigianii a

11A

Ent. aerogenes or K. pneumoniae subsp. pneumoniae

K. pneumoniae subsp. pneumoniae (97.6 %)

Good

K. pneumoniae subsp. pneumoniae a

11B

Erwinia cacticida

Buttiauxella agrestis (63 %)

Low

b

12

Erwinia cacticida or Erwinia dissolvens

Ent. sakazakii (51.1 %), Ent. amnigenus 1 (31.7 %)

Excellent identification to the genus

Contradiction (Enterobacter/Erwinia spp.)a

54A

Ent. cloacae or Ent. agglomerans

K. pneumoniae subsp. pneumoniae (98 %)

Good

c

54B

K. oxytoca

Ent. sakazakii (99.9 %)

Excellent

c

55A

Erwinia cacticida or Serratia entomophila

Pantoea spp. 2 (50.7 %), K. pneumoniae subsp. pneumoniae (30.4 %)

Low

b

55B

Ent. amnigenus, Ent. sakazakii or Ent. nimipressuralis

Ent. sakazakii (98.4 %)

Good

Ent. sakazakii a

56

K. oxytoca

Ent. sakazakii (99.9 %)

Excellent

c

82A

Pseudomonas spp. other than P. aeruginosa

Non fermentor spp. (32.1 %), Ochrobactrum anthropi (25.4 %), Bordetella/ Alcaligenes/ Moraxella spp. (24.4 %)

Low

b

  1. aMolecular identification was not deemed necessary
  2. bThe isolate was not properly identified by biochemical methods and therefore molecular identification was required
  3. cContradictory identification results using biochemical methods necessitate the use of molecular identification
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BMC Microbiology

ISSN: 1471-2180

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