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Table 3 Identification of Gram-negative isolates using biochemical methods

From: Microbiological testing of pharmaceuticals and cosmetics in Egypt

Isolate code Identification based on conventional biochemical tests Identification based on API 20E kit Final conclusion
Result (%ID) Level of discrimination
1 Erwinia cacticida or Erwinia dissolvens Ent. cloacae (94.3 %) Good Contradiction (Enterobacter/Erwinia spp.)a
8A Providencia alcalifaciens, Providencia rustigianii or Providencia stuartii Providencia alcalifaciens/rustigianii (72 %) Very good identification to the genus Providencia alcalifaciens/rustigianii a
11A Ent. aerogenes or K. pneumoniae subsp. pneumoniae K. pneumoniae subsp. pneumoniae (97.6 %) Good K. pneumoniae subsp. pneumoniae a
11B Erwinia cacticida Buttiauxella agrestis (63 %) Low b
12 Erwinia cacticida or Erwinia dissolvens Ent. sakazakii (51.1 %), Ent. amnigenus 1 (31.7 %) Excellent identification to the genus Contradiction (Enterobacter/Erwinia spp.)a
54A Ent. cloacae or Ent. agglomerans K. pneumoniae subsp. pneumoniae (98 %) Good c
54B K. oxytoca Ent. sakazakii (99.9 %) Excellent c
55A Erwinia cacticida or Serratia entomophila Pantoea spp. 2 (50.7 %), K. pneumoniae subsp. pneumoniae (30.4 %) Low b
55B Ent. amnigenus, Ent. sakazakii or Ent. nimipressuralis Ent. sakazakii (98.4 %) Good Ent. sakazakii a
56 K. oxytoca Ent. sakazakii (99.9 %) Excellent c
82A Pseudomonas spp. other than P. aeruginosa Non fermentor spp. (32.1 %), Ochrobactrum anthropi (25.4 %), Bordetella/ Alcaligenes/ Moraxella spp. (24.4 %) Low b
  1. aMolecular identification was not deemed necessary
  2. bThe isolate was not properly identified by biochemical methods and therefore molecular identification was required
  3. cContradictory identification results using biochemical methods necessitate the use of molecular identification