Fig. 5
Binding of NTHi to immobilized vitronectin in the presence of protein-E peptide. Human plasma vitronectin was bound to glass-bottomed dishes. NTHi (HUSM 0481) was incubated on the plate-bound vitronectin for 30 minutes, and then the dishes were washed three times. In some experiments, plate-bound vitronectin was pretreated with protein-E peptide (PE84–108) before incubation with NTHi. BSA was used as a negative control. NTHi was stained with LIVE/DEAD®, and viable NTHi is stained green. a Representative fluorescent micrographs of NTHi incubated on plate-bound vitronectin that was untreated or pretreated with PE84–108. White bars represent 10 μm. b The number of attached NTHi per field at 1,000 × magnification that was pretreated with increasing concentrations of PE84–108. Error bars represent SEM in three independent experiments that gave similar results. **p < 0.01