Molecular epidemiology of carbapenem resistant Enterobacteriaceae in Valle d’Aosta region, Italy, shows the emergence of KPC-2 producing Klebsiella pneumoniae clonal complex 101 (ST101 and ST1789)

Table 2 Conjugative transfer of carbapenem resistance in Enterobacteriacea

Strain number	Donor Genotype^a	Transconjugant Genotype^a	Frequency of transfer^b	Replicon typing^c	ESBL^d	Carbapenemase
5:J53	ST1789/A	ST10/d	8.3×10⁻⁴	FII	CTX-M-1 group	KPC-2
8:J53	ST101/A1	ST10/d	1.4 × 10⁻⁶	FII	CTX-M-1 group	KPC-2
2:J53	ST512/B	ST10/d	6.8 × 10⁻⁵	FIIk	-	KPC-3
12:J53	ST512/C	ST10/d	2.5 × 10⁻⁷	FIIk	-	KPC-3
24:J53	ST405/D	ST10/d	1.6 × 10⁻⁵	FIIk	-	KPC-3
6:J53	ST131/a	ST10/d	2.5 × 10⁻²	N	-	VIM-1
J53	ST10/d	ST10/d	-	-	-	-

^aThe genotypes of the strains were identified by MLST sequencing types (STs) and PFGE types. Different MLST schemes were used for K. pneumoniae and E. coli as described in the methods section
^bMating rate was calculated as frequency of carbapenem resistant transconjugants for recipient cell resistant to sodium-azide in filter mating experiments
^cReplicon typing was performed as describe in the materials and method section
^dESBL (extended-spectrum beta-lactamase)

ISSN: 1471-2180