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Fig. 2 | BMC Microbiology

Fig. 2

From: Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1–4

Fig. 2

Agarose gel electrophoresis and restriction analysis of DENV serotype-specific RT-LAMP assay products on a 2 % agarose gel. M, DL1000 DNA ladder (TAKARA, Japan); 1, DENV1 RT-LAMP amplification; 2, XbaI restriction enzyme digestion of DENV1 RT-LAMP product, 190,235,280 bp respectively; 3, DENV2 RT-LAMP amplification; 4, SpeI restriction enzyme digestion of DENV2 RT-LAMP product, 190,205,220 bp respectively; 5, DENV3 RT-LAMP assay amplification; 6, BglII restriction enzyme digestion of DENV3 RT-LAMP product, 145,185,210 bp respectively; 7, DENV4 RT-LAMP assay amplification; 8, AbaI restriction enzyme digestion of DENV4 RT-LAMP product, 180,210 bp respectively

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