Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources

Table 2 The information of primers and housekeeping genes for MLST of L. plantarum

Gene	Template Length (bp)	Gene function	PCR primer	Sequences (5’-3’)	Annealing Temperature
pheS	717	Phenylalanyl-tRNA synthase alpha subunit	pheS _primerF	CCGTGAAGAACTGGAACA	49 °C
pheS	717	Phenylalanyl-tRNA synthase alpha subunit	pheS _primerR	CCTAACCCAAAGGCAAAA	49 °C
pyrG	668	CTP synthase	pyrG_primerF	AGTGATTTAGGTTCCGACAA	52 °C
pyrG	668	CTP synthase	pyrG_primerR	TGCATTCCCAAGCAGATA	52 °C
uvrC	763	UvrABC system protein C	uvrC _primerF	GATCATTTATGTGGGTAAGGC	53 °C
uvrC	763	UvrABC system protein C	uvrC _primerR	TGACACTACTGGGAACAAGC	53 °C
recA	689	Recombinase A	recA_primerF	TTTTAGTTGTTGACTCGGTGGC	58 °C
recA	689	Recombinase A	recA_primerR	TTCCGCTGGTGTCGCTTT	58 °C
clpX	563	ATP-dependent Clp protease subunit X	clpX_primerF	ATCGCCAAGAAGAGTGAA	52 °C
clpX	563	ATP-dependent Clp protease subunit X	clpX_primerR	ATAATCGAGCGTAGACCC	52 °C
murC	641	UDP-N-acetyl muramate-alanine ligase	murC_primerF	TATCGCTCCCACCAGTTA	51 °C
murC	641	UDP-N-acetyl muramate-alanine ligase	murC_primerR	CGGCCAAGATTTCCTTAT	51 °C
groEL	737	60 kDa chaperonin	groEL_primerF	CGGCTACTTATCACAATACA	58 °C
groEL	737	60 kDa chaperonin	groEL_primerR	GCCTTCTAAACCAGCATT	58 °C
murE	750	UDP-N-acetylmuramyl tripeptide synthase	murE_primerF	ACTAATAAGGTCGCTGTTCTG	55 °C
murE	750	UDP-N-acetylmuramyl tripeptide synthase	murE_primerR	TTTAGCGGCTTCTTCACT	55 °C

ISSN: 1471-2180