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Fig. 7 | BMC Microbiology

Fig. 7

From: Characterization of proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans and its functional analyses in S. Cerevisiae

Fig. 7

Growth curves of various yeast strains expressing CaPCNA: a. Genomic POL30 deletion yeast strains containing YEP-ADH1p-ScPCNA (−−▲--), YCP-ADH1p-CaPCNA (−−■--) , YEP-ADH1p-CaPCNA (−−▼--), and YCP-ScPCNA gene with DE41,42AA (--●--) were grown in 100 ml YPD liquid medium at 30 °C and absorbance at 600 nm were measured at regular intervals. The plot was an average three sets of experiment. b. Total RNA from the above strains were isolated and semi-quantitive Reverse Trascriptase PCR was carried out. The PCR products were analyzed in a 1 % agarose gel electrophoresis. Lane 1, 1 KB DNA ladder; Lane 2 c-DNAs of ScPCNA DE41,42AA and ScPolη; Lane 3 c-DNAs of CaPCNA and ScPolη; Lane 4 c-DNAs of ScPCNA and ScPolη; Lane 5 c-DNAs of CaPCNA and ScPolη; Lane 6 no c-DNA control reaction.

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BMC Microbiology

ISSN: 1471-2180

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