Fig. 3From: Characterization of proliferating cell nuclear antigen (PCNA) from pathogenic yeast Candida albicans and its functional analyses in S. Cerevisiae Purification and biochemical analysis of PCNAs. The wild type and mutant PCNAs were purified and ~1 μg of each protein was analyzed. a . Purified proteins were resolved in SDS-12 % polyacrylamide gel. Lanes 1, Molecular weight standards; 2, ScPCNA; 3, CaPCNA; and 4, CaPCNA G178S. c. Purified proteins were resolved in 12 % non-denaturing polyacrylamide gel. Lanes 1, CaPCNA; 2, CaPCNA G178S; and 3. ScPCNA. c. Size exclusion chromatograms of wild type (blue) and G178S mutant (red) CaPCNAs with the peak fractionation at ~1.6 ml and ~2.3 ml elution volume, respectively, were shown. d. Glutaraldehyde crosslinked 20 μg of CaPCNA (lane 2) or CaPCNA G178S (lane 3) or ScPCNA G178S (lane 4) proteins were resolved in SDS-12 % polyacrylamide gel. Monomer (~29 kDa) and oligomeric PCNAs (~60-180 kDa) are marked. Lane 1, Molecular weight standards.Back to article page