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Table 3 Quantitative detection of C. perfringens in stool samples from healthy infants and young adults by Amp-qPCR assays targeting 16S rRNA, plc and cpe genes

From: Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes

Target

Infant aged 6 months

Young adult

(n = 124)

(n = 221)

Bacterial count (log10 bacterial cells/g feces)a

Detection rate (%)

Bacterial count (log10 bacterial cells/g feces)a

Detection rate (%)

16S rRNA gene

6.4 ± 1.3

35

4.8 ± 1.2b

31

plc

6.0 ± 1.5

36

4.8 ± 1.2b

33

cpe

5.9 ± 1.9

10

4.8 ± 0.8

1c

  1. aData are expressed as the means and standard deviations
  2. b p < 0.05 (vs. Infant aged 6 months), Mann–Whitney U test
  3. c p < 0.05 (vs. Infant aged 6 months), Fisher’s exact probability test
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BMC Microbiology

ISSN: 1471-2180

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