Sensitive quantification of Clostridium perfringens in human feces by quantitative real-time PCR targeting alpha-toxin and enterotoxin genes

Table 1 C. perfringens targeted primers used in this study

Target	Standard strain	Primer	Sequence (5' - 3')	Product size (bp)	Annealing temp (°C)	Reference
plc gene	C. perfringens ATCC 13124^T	Cper-plc508-F	CCGTTGATAGCGCAGGACA
		Cper-plc508-R	CCCAACTATGACTCATGCTAGCA	219	60	This study
cpe gene	C. perfringens	GAP11	GGTTCATTAATTGAAACTGGTG	154	55	[37]
		GAP12	AACGCCAATCATATAAATTACAGC
16S rRNA gene	C. perfringens ATCC 13124^T	s-Clper-F	GGGGGTTTCAACACCTCC	170	60	[38, 74]
16S rRNA gene	C. perfringens ATCC 13124^T	ClPER-R	GCAAGGGATGTCAAGTGT	170	60	[38, 74]

ISSN: 1471-2180