Fig. 1From: Chromosomal integration vectors allowing flexible expression of foreign genes in Campylobacter jejuni Construction of expression vectors for integration onto the C. jejuni chromosome. Construction of the pCJC series of plasmids for integration in the C. jejuni 11168 pseudogene Cj0223. A fragment of Cj0223 cloned into pUC18 is interrupted by a chloramphenicol resistance cassette (cat) at a unique SpeI site. Promoter regions with Shine-Dalgarno (SD) sites were cloned into the XhoI site followed by insertion of gfp at the NdeI site. Three promoter regions were used: H. pylori ureI to create pCJC2, H. pullorum flaA to create pCJC3 and C. jejuni porA to create pCJC4Back to article page