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Table 1 Strains and plasmids used in this study

From: Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum

Strain or plasmid Relevant characteristics Reference or source
E. coli DH5α F thi-1 endA1 hsdR17(rm) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1 [59]
C. glutamicum strains   
 WT Wild-type strain ATCC 13032 American Type Culture Collection
 Tol1 Methanol tolerant strain derived from C. glutamicum WT carrying the vector pVWEx1 This study
 Tol2 Methanol tolerant strain derived from C. glutamicum WT carrying the vector pVWEx1 This study
 ΔadhA in-frame deletion of the adhA gene (cg3107) of C. glutamicum WT [21]
 ΔramA in-frame disruption of the ramA gene (cg2831) of C. glutamicum WT [55]
 T0198 point mutation cg0198A19V from strain Tol1 in the cg0198 gene of C. glutamicum WT This study
 T0755 point mutation cg0755A165T from strain Tol1 in the cg0755 gene of C. glutamicum WT This study
 T1245 point mutation cg1245L328S from strain Tol1 in the cg1245 gene of C. glutamicum WT This study
 T2204 point mutation cg2204D67H from strain Tol1 in the cg2204 gene of C. glutamicum WT This study
 T2840 point mutation cg2840Q342* from strain Tol1 in the cg2840 gene of C. glutamicum WT This study
 T0755 + 2840 Combination of the point mutations from strain Tol1 in the cg0755 and cg2840 genes of C. glutamicum WT This study
 Δcat in-frame deletion of the cat gene (cg2840) of C. glutamicum WT lacking both the N- and C-terminal regions of Cat [41]
Plasmids   
 pK19mobsacB Kmr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) [65]
 pK19mobsacB-T0198 Kmr, pK19mobsacB with the construct for a base exchange from C to T at position 56 in cg0198 This study
 pK19mobsacB-T0755 Kmr, pK19mobsacB with the construct for a base exchange from G to A at position 493 in cg0755 This study
 pK19mobsacB-T1245 Kmr, pK19mobsacB with the construct for a base exchange from T to C at position 983 in cg1245 This study
 pK19mobsacB-T2204 Kmr, pK19mobsacB with the construct for a base exchange from G to C at position 199 in cg2204 This study
 pK19mobsacB-T2840 Kmr, pK19mobsacB with the construct for a base exchange from C to T at position 1024 in cg2840 This study
 pVWEx1 Kmr; C. glutamicum/E. coli shuttle vector (P tac lacI q oriV C.g . oriV E.c .) [71]