Table 1 Strains and plasmids used in this study
From: Identification of two mutations increasing the methanol tolerance of Corynebacterium glutamicum
Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
E. coli DH5α | F− thi-1 endA1 hsdR17(r−m−) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 relA1 | [59] |
C. glutamicum strains | Â | Â |
 WT | Wild-type strain ATCC 13032 | American Type Culture Collection |
 Tol1 | Methanol tolerant strain derived from C. glutamicum WT carrying the vector pVWEx1 | This study |
 Tol2 | Methanol tolerant strain derived from C. glutamicum WT carrying the vector pVWEx1 | This study |
 ΔadhA | in-frame deletion of the adhA gene (cg3107) of C. glutamicum WT | [21] |
 ΔramA | in-frame disruption of the ramA gene (cg2831) of C. glutamicum WT | [55] |
 T0198 | point mutation cg0198A19V from strain Tol1 in the cg0198 gene of C. glutamicum WT | This study |
 T0755 | point mutation cg0755A165T from strain Tol1 in the cg0755 gene of C. glutamicum WT | This study |
 T1245 | point mutation cg1245L328S from strain Tol1 in the cg1245 gene of C. glutamicum WT | This study |
 T2204 | point mutation cg2204D67H from strain Tol1 in the cg2204 gene of C. glutamicum WT | This study |
 T2840 | point mutation cg2840Q342* from strain Tol1 in the cg2840 gene of C. glutamicum WT | This study |
 T0755 + 2840 | Combination of the point mutations from strain Tol1 in the cg0755 and cg2840 genes of C. glutamicum WT | This study |
 Δcat | in-frame deletion of the cat gene (cg2840) of C. glutamicum WT lacking both the N- and C-terminal regions of Cat | [41] |
Plasmids | Â | Â |
 pK19mobsacB | Kmr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZα) | [65] |
 pK19mobsacB-T0198 | Kmr, pK19mobsacB with the construct for a base exchange from C to T at position 56 in cg0198 | This study |
 pK19mobsacB-T0755 | Kmr, pK19mobsacB with the construct for a base exchange from G to A at position 493 in cg0755 | This study |
 pK19mobsacB-T1245 | Kmr, pK19mobsacB with the construct for a base exchange from T to C at position 983 in cg1245 | This study |
 pK19mobsacB-T2204 | Kmr, pK19mobsacB with the construct for a base exchange from G to C at position 199 in cg2204 | This study |
 pK19mobsacB-T2840 | Kmr, pK19mobsacB with the construct for a base exchange from C to T at position 1024 in cg2840 | This study |
 pVWEx1 | Kmr; C. glutamicum/E. coli shuttle vector (P tac lacI q oriV C.g . oriV E.c .) | [71] |