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Fig. 1 | BMC Microbiology

Fig. 1

From: A GntR family transcription factor positively regulates mycobacterial isoniazid resistance by controlling the expression of a putative permease

Fig. 1

The effect of Ms0535 on the drug resistance of M. smegmatis and a sequence alignment of the Ms0535 C-terminal domain. (a) Isoniazid (INH)-resistance of the M. smegmatis (Msm) strain overexpressing Ms0535. Msm/pMV261 and Msm/pMV261-Ms0535 were grown on medium containing 30 μg/mL kanamycin for 36 h. Then, different concentrations of freshly grown bacteria were streaked onto 7H10 plates containing kanamycin (30 μg/mL) and INH (20 μg/mL). The control plate did not contain INH. (b) Analysis of the structural characteristics of Ms0535. The Ms0535 amino acid sequence was analyzed using the online tool SWISS-MODEL. The N-terminal region of Ms0535 contains a winged helix-turn-helix GntR DNA-binding domain, while its C-terminus contains a domain that is similar to that of the FCD family. Amino acid residues present in α-helices are highlighted in gray, and spaces in the consensus sequence denote insertions within the alignment

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BMC Microbiology

ISSN: 1471-2180

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