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Table 1 Strains and plasmids used in this study

From: Molecular study on the carAB operon reveals that carB gene is required for swimming and biofilm formation in Xanthomonas citri subsp. citri

Strain or plasmid Relevant characteristics Source
strains
Escherichia coli
 DH5 α Φ901acZΔm15, recA1 Invitrogen
Xanthomonas citri subsp. citri
Xac 29-1 Wild-type This lab
Xac 29-1/pUGPcarAB Xac 291 carrying pUGPcarAB This work
ΔcarA A carA knock-out mutant of strain Xac 29-1 [9]
ΔcarB A carB knock-out mutant of strain Xac 29-1 [9]
 CΔcarA Gmr, ΔcarB harboring pBBR1MCS-5 expressing carA gene under wxacO promoter [9]
 CΔcarB Gmr, ΔcarB harboring pBBR1MCS-5 expressing carB gene under wxacO promoter [9]
Plasmids
 pUFR034 Kmr, IncW, Mob(p), Mob + , LacZa +, PK2 replicon, cosmid [29]
 pUGPcarAB Kmr, carAB operon promoter and gusA gene fused in pUFR034 vector This work