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Table 1 Kinetic parameters of R. solanacearum FabG proteins

From: Ralstonia solanacearum fatty acid composition is determined by interaction of two 3-ketoacyl-acyl carrier protein reductases encoded on separate replicons

 

Vmax (nmol/min × μg)

Km (μmol/L)

Kcat (sec−1)

AACa

NADPH

AAC a

NADPH

AAC

NADPH

EcFabG

97.02 ± 8.5

3.54 ± 0.5

4372.6 ± 105.1

5.41 ± 1.3

45.28 ± 4.3

1.65 ± 0.43

RsFabG1

4.70 ± 1.3

1.50 ± 0.5

313.28 ± 14.5

25.75 ± 3.8

2.19 ± 078

0.71 ± 0.24

RsFabG2

ND b

ND

ND

ND

ND

ND

  1. a AAC denotes acetoacetyl-CoA
  2. b ND, could not be detected
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BMC Microbiology

ISSN: 1471-2180

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