Table 4 Strains and plasmids used in this study
Strain | Genotype | Reference/Source |
MG1655 (PL2036) | F- lambda-ilvG-rfb-50 rph-1 | Â |
DRC14 | MC4100 ftsZ84(ts) leu::Tn10 | John Beckwith |
ftsZ84 (PL2452) | MG1655, leu82::Tn10Â ftsZ84(ts) | Â |
ftsZ84*F39L (HA209) | MG1655, leu82::Tn10Â ftsZ84 *F39L | This study |
ftsZ84 *M206I (HA211) | MG1655, leu82::Tn10 ftsZ84 *M206I | This study |
ftsZ84 *V293I (HA213) | MG1655, leu82::Tn10 ftsZ84 *V293I | This study |
HA223 | MG1655, leu82::Tn10Â ftsZ84(ts), pBAD-ftsW | This study |
zapA (HA235) | MG1655, zapA::kan | This study |
ftsZ84, zapA (HA241) | ftsZ84, zapA::kan | This study |
ftsZ84*F39L, zapA (HA267) | ftsZ84 *F39L, zapA::kan | This study |
ftsZ84*M206I, zapA (HA245) | ftsZ84 *M206I, zapA::kan | This study |
ftsZ84*V293I, zapA (HA251) | ftsZ84*V293I, zapA::kan | This study |
HA255 | MG1655, pKG110-zapA | This study |
HA257 | ftsZ84, pKG110-zapA | This study |
CW206 | MG1655, pQE80-H6-zapA | This study |
HA364 | ftsZ84, pQE80-H6-zapA | This study |
Plasmid/Strain harboring plasmid | Genotype | Reference |
pPJ2 (PL3142) | pET21b(+)-ftsZ | Buske and Levin, 2012 |
HA146 | pET21b(+)-ftsZ84 | This study |
HA149 | pET21b(+) -ftsZ84 *F39L | This study |
HA147 | pET21b(+)-ftsZ84 *M206I | This study |
HA142 | pET21b(+)-ftsZ84*V293I | This study |
HA165 | pET21b(+)-ftsZ *F39L | This study |
HA157 | pET21b(+)-ftsZ *M206I | This study |
HA166 | pET21b(+)-ftsZ*V293I | This study |
PL3463 | pBAD24-ftsW | This study |
HA255 | pKG110-zapA | Haeusser et al., 2014 |
HA364 | pQE80-H6-zapA | Dajkovic et al., 2001 |