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Fig. 6 | BMC Microbiology

Fig. 6

From: Mutations in the bacterial cell division protein FtsZ highlight the role of GTP binding and longitudinal subunit interactions in assembly and function

Fig. 6

zapA deletions reduce ftsZ84* viability under nonpermissive conditions. a Plating efficiency of WT, ftsZ84, and ftsZ84* strains when grown in permissive conditions and plated onto nonpermissive conditions. b Plating efficiency assays of zapA deletions in wild-type, ftsZ84, and ftsZ84* suppressor strains. Tenfold dilutions of cells cultured in permissive conditions were plated under nonpermissive conditions. c zapA overexpression from a sodium salicylate inducible zapA plasmid (pKG110-zapA) does not restore viability to ftsZ84 and makes ftsZ84* suppressor strains more sensitive when plated under nonpermissive conditions with 0.5 μM of sodium salicylate. Identical results were obtained when using the IPTG inducible zapA vector (pQE80-H6-zapA) in ftsZ84 (see Additional file 1). b and c These experiments were performed at least 3 times with equivalent results

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BMC Microbiology

ISSN: 1471-2180

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