Fig. 5
ftsZ84* suppressor mutations do not restore assembly in vitro. a-b 90° angle light scattering data from FtsZ (blue), FtsZ84 (red), FtsZ84*F39L (green), FtsZ84*M206I (purple), and FtsZ84*V293I (aqua) a 50 mM MES, pH 6.5, 50 mM KCl, 2.5 mM MgCl 2 , 1 mM EGTA or b 50 mM MES, pH 6.5, 50 mM KCl, 10 mM MgCl 2 , 1 mM EGTA (methods). After establishing baseline, assembly was initiated by the addition of 1 mM GTP. c 90° angle light scattering data from suppressor mutations alone (in the absence of ftsZ84(G105S)). FtsZ (blue), FtsZ84 (red), FtsZ*F39L (green), FtsZ*M206I (purple), and FtsZ*V293I (aqua) Reaction conditions same as in (a). d Electron micrographs of FtsZ assembled in 50 mM KCl or 250 mM KCl (50 mM MES, pH 6.5, 50 mM KCl, 2.5 mM MgCl2, 1 mM EGTA) Bar = 100 nm