Skip to main content

Table 2 Specificity of the developed bead-based Luminex immunoassay

From: Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species

Bacterial species Strain O-Antigen Luminex assay (mean fluorescence intensity)
Brucella spp. (biotype)
 B. abortus (1) NCTC 10093 544 A1,3 13107
  19S A1,3 11300
  A146-10 A1,3 5607
 B. abortus (3) A104-10 Uckermark A1,3 3172
 B. canis NCTC 10854 RM-666 R2,3 33
 B. ovis CNCTC 6741 R2,3 23
 B. melitensis (1) NCTC 10094 16 M M1,3 25860
  ICM 3.33 M1,3 13129
  ICM 583/2003 M1,3 16972
  ICM 91/2004 M1,3 15030
  102A01C2F M1,3 22285
  A146-13 M1,3 11407
  A104-11 Tgb. Nr. 117518 M1,3 5977
 B. melitensis (2) A104-12 799/97, B3898 A1,3 2958
 B. melitensis (3) A104-13 210739, Mainz AM1,3 1884
 B. suis (1) NCTC 10316 1330 A1,3 5167
 B. suis (2) A 104–14 Rostock A1,3 10316
 B. neotomae A148-7 5 K33 A1,3 1875
Other bacteria   
 F. tularensis tularensis ATCC 6223 D4 18
 F. tularensis holarctica NCTC 10857 D4 22
 B. anthracis (spores) Böhm 73202.2000 (PX02) D5 19
 S. typhimurium ATCC 14028 D6 19
 Y. enterocolitica 310 (IT2, ST9) O9 A7,8,9 1291
 Y. pestis CO92 R10 20
 O. anthropi ATCC 49188 D11 20
 E. coli O157, 15326 A7,12 22
 B. mallei NCTC 03709 106 D13 18
 B. pseudomallei H05410-0490 D14 20
 V. cholera O1, ATCC 14734 A15 24
  1. Luminex LOD was defined as two times the mean fluorescence intensity of the blank (mean blank = 20) and used as the threshold for positive results. Values in bold indicate positive results. Classification of O-antigens [4, 2629, 4150]: A = α1,2-linked 4-amino-4,6-dideoxy-α-D-mannopyranosyl subunits, M = α1,3-linked and α1,2-linked 4,6-dideoxy-4-formamido-α-D-mannopyranosyl residues, D = different O-antigen structure compared to Brucella, R = ‘rough’ LPS (no O-antigen). Meikle et al. 19891, Adone et al. 20112, Corbel 20063, Wang et al. 20114, Crich and Vinogradova 20075, Watson et al. 19926, Perry et al. 19867, Caroff et al. 19848, Bundle et al. 19849, Skurnik et al. 200010, Velasco et al. 199611, Perry and Bundle 199012, Burtnick et al. 200213, Perry et al. 199514, Kenne et al. 198215