Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by protein kinase A

Table 3 Proteins identified in the secretome of C. neoformans collected at 96 hpi from cells grown in Pka1-induced (galactose-containing medium) conditions

GO categories^a	Accession number	Protein name	Fold change^b	Std. Dev.
Carbohydrate catabolic process
	CNAG_02189	α-Amylase	1.061	0.080
GTP catabolic process
	CNAG_06125	Translation elongation factor 1 α	0.462	0.555
Carbohydrate metabolic process
	CNAG_04245	Chitinase	0.621	0.562
	CNAG_06501	1,3-β-glucanosyltransferase	1.101	0.140
	CNAG_01239	Chitin deacetylase	2.659	1.996
	CNAG_02860	Endo-1,3(4)-β-glucanase	3.210	1.665
Protein ubiquitination
	CNAG_01920	Polyubiquitin	0.509	0.501
ATP hydrolysis coupled proton transport
	CNAG_05750	ATPase α subunit	1.785	1.765
Dephosphorylation
	CNAG_02944	Acid phosphatase	0.233	0.061
Oxidation-reduction process
	CNAG_01019	Cu/Zn superoxide dismutase	0.350	0.142
	CNAG_03465	Laccase	1.000	0.107
Proteolysis
	CNAG_00919	Carboxypeptidase D	5.770	3.708
Response to stress
	CNAG_01750	Chaperone	>10	>10
Unknown/Unclassified
	CNAG_04291	Glycosyl-hydrolase	0.989	0.959
	CNAG_00407	Glyoxal oxidase	1.209	0.648
	CNAG_06267	Rds1 protein	2.834	0.518
	CNAG_01653	Cytokine-inducing glycoprotein	2.951	2.753
	CNAG_04753	Lactonohydrolase	>10	>10
Hypothetical
	CNAG_06109	Conserved hypothetical protein	0.463	0.431
	CNAG_05893	Conserved hypothetical protein	1.062	0.307
	CNAG_05312	Conserved hypothetical protein	3.737	2.342

^aProteins were categorized based on GO terms associated with their biological classification
^bFold change is reported as the average quantification for P _GAL7 ::PKA1 vs. WT, ± standard deviation

ISSN: 1471-2180