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Fig. 8 | BMC Microbiology

Fig. 8

From: Secretome profiling of Cryptococcus neoformans reveals regulation of a subset of virulence-associated proteins and potential biomarkers by protein kinase A

Fig. 8

Detection of Pka1-regulated proteins in mouse bronchoalveolar lavage samples. Chromatographic representation of the most abundant peptide and its transition identified from isotopically-labaled peptide or natural peptide for each of the following proteins: (a) Cig1 (CNAG_01653), (b) α-Amylase (CNAG_02189), (c) Glyoxal oxidase (CNAG_00407), (d) Hypothetical protein (CNAG_05312). Black indicates isotopically-labeled peptide; red indicates natural peptide. e Quantification of proteins identified in the mouse BAL samples based the area under the curve for the isotopically-labeled peptide versus the natural peptide, for Cig1, α-amylase, glyoxal oxidase, and hypothetical (CNAG_05312) proteins. The avergae (± S.D.) amount of peptide present in the sampleis reported. Five micrograms of total protein were used for the assays and all assays were performed in triplicate

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BMC Microbiology

ISSN: 1471-2180

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