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Table 2 Candida albicans proteins with the previously confirmed binding affinity to human fibronectin, vitronectin and laminin

From: Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts

Abbreviation Protein name Method used for protein identification and confirmation of binding to ECMPs Reference
Fibronectin-binding proteins
Als1 Agglutinin-like sequence protein 1 expression in Saccharomyces cerevisiae followed by binding to magnetic beads coated with fibronectin [38]
expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements [39]
Als3 Agglutinin-like sequence protein 3 expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
expression in S. cerevisiae followed by microtiter plate-based assay [40]
Als5 Agglutinin-like sequence protein 5 expression in S. cerevisiae followed by binding to microplate-immobilized fibronectin [16]
binding of protein fragments—Als5 1–431 and Als5 1-664—to fibronectin-coated microtiter plate wells [41]
Hwp1 Hyphal cell wall protein 1 expression in S. cerevisiae followed by microtiter plate-based assay [40]
Tdh3 Glyceraldedyhde-3-phosphate dehydrogenase immunoaffinity purification of Tdh3 followed by ligand Western Blot assay [18]
Adh1 Alcohol dehydrogenase screening of C. albicans cDNA library with polyclonal antibodies against receptor for fibronectin [17]
Vitronectin-binding proteins
Adh1 Alcohol dehydrogenase screening of C. albicans cDNA library with polyclonal antibodies against receptor for vitronectin [17]
Gpm1 Phosphoglycerate mutase enzyme-linked ligand sorbent assay (ELISA) with microplate-immobilized recombinant Gpm1 [45]
Laminin-binding proteins
Als1 Agglutinin-like sequence protein 1 expression in S. cerevisiae followed by binding to magnetic beads coated with laminin [38]
expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
expression of the Als1 N-terminal part in S. cerevisiae followed by its purification and surface plasmon resonance measurements [39]
Als3 Agglutinin-like sequence protein 3 expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
Als5 Agglutinin-like sequence protein 5 expression in S. cerevisiae followed by binding to microplate-immobilized laminin [16]
Tdh3 Glyceraldedyhde-3-phosphate dehydrogenase immunoaffinity purification of Tdh3 followed by ligand Western Blot assay [18]