Fig. 5

Modulation of the proinflammatory response triggered by LPS in RAW264.7 mouse macrophages. RAW264.7 cells were stimulated with L. helveticus NS8 (bacteria: cell ratio of 100:1) or its S-layer protein (10 μg/ml), with or without co-incubation of 1 μg/mL LPS for 4 h. Expression profiles of IL10 (a), IL-12p70 (b), and TNF-α (c) were examined by quantitative real-time PCR, indicated as the relative levels to the induction level of the control, which was set at a value of 1. Results are the means ± SD of representative of three independent experiments. Significant differences indicated as: **, P < 0.01; *, P < 0.05